Stephensi Multiplexing Analysis
Homing Rates
Means and 95%CI for homing plots
Mean values
| Transgenic | wild-type | cd225 | cd384 |
|---|---|---|---|
| cdg225 | 0.92 (0.63-0.99) |
- | 0.91 (0.58-0.99) |
| cdg338-384 | 1 (0.97-1) |
- | 0.96 (0.78-0.99) |
| cdg384_del | 0.96 (0.87-0.99) |
- | - |
| cdg384 | 0.99 (0.98-1) |
1 (0.98-1) |
0.51 (0.14-0.87) |
Summary model table
| Homing Rate | |||
|---|---|---|---|
| Predictors | Odds Ratios | 95% CI | P-Value |
| (Intercept) | 11.63 | 1.71 – 78.99 | 0.012 |
| gRNA_typecdg338-384 | 18.95 | 1.22 – 293.69 | 0.035 |
| gRNA type [cdg384_del] |
2.36 | 0.22 – 24.75 | 0.475 |
| gRNA type [cdg384] |
15.02 | 1.71 – 132.34 | 0.015 |
| pre cut [cd225] |
1.40 | 0.24 – 8.01 | 0.708 |
| pre cut [cd384] |
0.82 | 0.05 – 12.49 | 0.886 |
| gRNA_typecdg338-384:pre_cutcd384 | 0.14 | 0.00 – 6.38 | 0.309 |
| gRNA type [cdg384] × pre cut [cd384] |
0.01 | 0.00 – 0.24 | 0.006 |
| Random Effects | |||
| σ2 | 4.93 | ||
| τ00 id:group_letter | 1.64 | ||
| τ00 group_letter | 0.86 | ||
| ICC | 0.15 | ||
| N id | 328 | ||
| N group_letter | 13 | ||
| Observations | 328 | ||
| Marginal R2 / Conditional R2 | 0.325 / 0.425 | ||
Contrasts
| contrast | odds.ratio | SE | df | null | z.ratio | p.value |
|---|---|---|---|---|---|---|
| (cdg225 wild-type) / (cdg338-384 wild-type) | 0.05 | 0.07 | Inf | 1 | -2.1 | 0.04 |
| (cdg225 wild-type) / (cdg384_del wild-type) | 0.42 | 0.51 | Inf | 1 | -0.71 | 0.48 |
| (cdg225 wild-type) / (cdg384 wild-type) | 0.07 | 0.07 | Inf | 1 | -2.44 | 0.01 |
| (cdg225 wild-type) / (cdg384 cd225) | 0.05 | 0.06 | Inf | 1 | -2.49 | 0.01 |
| (cdg225 wild-type) / (cdg225 cd384) | 1.22 | 1.7 | Inf | 1 | 0.14 | 0.89 |
| (cdg225 wild-type) / (cdg338-384 cd384) | 0.48 | 0.66 | Inf | 1 | -0.54 | 0.59 |
| (cdg225 wild-type) / (cdg384 cd384) | 11 | 15.09 | Inf | 1 | 1.75 | 0.08 |
| (cdg338-384 wild-type) / (cdg384_del wild-type) | 8.04 | 9.77 | Inf | 1 | 1.72 | 0.09 |
| (cdg338-384 wild-type) / (cdg384 wild-type) | 1.26 | 1.42 | Inf | 1 | 0.21 | 0.84 |
| (cdg338-384 wild-type) / (cdg384 cd225) | 0.9 | 1.11 | Inf | 1 | -0.08 | 0.93 |
| (cdg338-384 wild-type) / (cdg225 cd384) | 23.12 | 32.51 | Inf | 1 | 2.23 | 0.03 |
| (cdg338-384 wild-type) / (cdg338-384 cd384) | 9.03 | 12.56 | Inf | 1 | 1.58 | 0.11 |
| (cdg338-384 wild-type) / (cdg384 cd384) | 208.5 | 289.56 | Inf | 1 | 3.84 | 0 |
| (cdg384_del wild-type) / (cdg384 wild-type) | 0.16 | 0.14 | Inf | 1 | -2.13 | 0.03 |
| (cdg384_del wild-type) / (cdg384 cd225) | 0.11 | 0.11 | Inf | 1 | -2.17 | 0.03 |
| (cdg384_del wild-type) / (cdg225 cd384) | 2.87 | 3.47 | Inf | 1 | 0.87 | 0.38 |
| (cdg384_del wild-type) / (cdg338-384 cd384) | 1.12 | 1.34 | Inf | 1 | 0.1 | 0.92 |
| (cdg384_del wild-type) / (cdg384 cd384) | 25.92 | 30.79 | Inf | 1 | 2.74 | 0.01 |
| (cdg384 wild-type) / (cdg384 cd225) | 0.72 | 0.64 | Inf | 1 | -0.37 | 0.71 |
| (cdg384 wild-type) / (cdg225 cd384) | 18.33 | 20.52 | Inf | 1 | 2.6 | 0.01 |
| (cdg384 wild-type) / (cdg338-384 cd384) | 7.15 | 7.89 | Inf | 1 | 1.78 | 0.07 |
| (cdg384 wild-type) / (cdg384 cd384) | 165.25 | 181.37 | Inf | 1 | 4.65 | 0 |
| (cdg384 cd225) / (cdg225 cd384) | 25.58 | 31.43 | Inf | 1 | 2.64 | 0.01 |
| (cdg384 cd225) / (cdg338-384 cd384) | 9.99 | 12.11 | Inf | 1 | 1.9 | 0.06 |
| (cdg384 cd225) / (cdg384 cd384) | 230.68 | 278.78 | Inf | 1 | 4.5 | 0 |
| (cdg225 cd384) / (cdg338-384 cd384) | 0.39 | 0.54 | Inf | 1 | -0.68 | 0.5 |
| (cdg225 cd384) / (cdg384 cd384) | 9.02 | 12.44 | Inf | 1 | 1.59 | 0.11 |
| (cdg338-384 cd384) / (cdg384 cd384) | 23.1 | 31.59 | Inf | 1 | 2.3 | 0.02 |
Meiotic Drive data
| Meiotic Drive | |||
| Predictors | Odds Ratios | 95% CI | P-Value |
| (Intercept) | 0.98 | 0.89 – 1.08 | 0.751 |
| gRNA type [cdg384] |
0.95 | 0.84 – 1.08 | 0.434 |
| Observations | 32 | ||
| Transgenic | Estimate |
|---|---|
| cdg338-384 | 0.5 (0.47-0.52) |
| cdg384 | 0.48 (0.46-0.5) |
Indel formation
Frequency of missing guide sites
Venn diagram of missing guide sites
